Graduate School of Medicine / School of Medicine, Faculty of Medicine

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Injection & Bioresponse


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Professor TOMITA Haruyoshi, M.D., Ph.D.
Associate Professor HIRAKAWA Hidetada, Ph.D.
Assistant Professor KURUSHIMA Jun, Ph.D.
HASHIMOTO Yuusuke, M.D., Ph.D.

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1. Hashimoto Y, Taniguchi M, Uesaka K, Nomura T, Hirakawa H, Tanimoto K, Tamai K, Ruan G, Zheng B, Tomita H. Novel multidrug-resistant enterococcal mobile linear plasmid pELF1 encoding vanA and vanM gene clusters from a Japanese vancomycin-resistant enterococci isolate. Front Microbiol. 2019, 10, 2568, doi: 10.3389/fmicb.2019.02568.

2. Hirakawa H, Suzue K, Kurabayashi K, Tomita H. The Tol-Pal system of uropathogenic Escherichia coli is responsible for optimal internalization into and aggregation within bladder epithelial cells, colonization of the urinary tract of mice, and bacterial motility. Front Microbiol. 2019, 10, 1827, doi: 10.3389/fmicb.2019.01827.

3. Hirakawa H, Takita A, Kato M, Mizumoto H, Tomita H. Roles of CytR, an anti-activator of cyclic-AMP receptor protein (CRP) on flagellar expression and virulence in uropathogenic Escherichia coli. Biochem Biophys Res Commun. 2020, 521 555-561, doi: 10.1016/j.bbrc.2019.10.165.

4. Hashimoto Y, Kurushima J, Nomura T, Tanimoto K, Tamai K, Yanagisawa H, Shirabe K, Ike Y, and Tomita H. Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan. BMC Microbiology 18:213, 2018

5. Hirakawa H, Kurabayashi K, Tanimoto K, and Tomita H. Oxygen Limitation Enhances the Antimicrobial Activity of Fosfomycin in Pseudomonas aeruginosa Following Overexpression of glpT Which Encodes Glycerol-3-Phosphate/Fosfomycin Symporter. Frontiers in Microbiology 9:1950, 2018

6. Nomura T, Hashimoto Y, Kurushima J, Hirakawa H, Tanimoto K, Zhneg B, Ruan G, Xue F, Liu J, Hisatsune J, Sugai M, and Tomita H. New colony multiplex PCR assays for the detection and discrimination of vancomycin-resistant enterococcal species. Journal of Microbiological Methods 145:69-72, 2018

7. Todokoro D, Suzuki T, Kobayakawa S, Tomita H, Ohashi Y, Akiyama H. Postoperative Enterococcus faecalis endophthalmitis: virulence factors leading to poor visual outcome. Jpn J Ophthalmol. 61(5):408-414, 2017

8. Kurabayashi K, Tanimoto K, Tomita H, Hirakawa H. Cooperative Actions of CRP-cAMP and FNR Increase the Fosfomycin Susceptibility of Enterohaemorrhagic Escherichia coli (EHEC) by Elevating the Expression of glpT and uhpT under Anaerobic Conditions.

Front Microbiol. 8:426, 2017

9. Kurabayashi K, Agata T, Asano H, Tomita H, Hirakawa H. Fur Represses Adhesion to, Invasion of, and Intracellular Bacterial Community Formation within Bladder Epithelial Cells and Motility in Uropathogenic Escherichia coli  Infection and Immunity 84(11):3220-3231,2016

10. Kurushima J, Ike Y, Tomita H. Partial Diversity Generates Effector Immunity Specificity of the Bac41-Like Bacteriocins of Enterococus faecalis Clinical Strains Journal of Bacteriology 198(17):2379-2390,2016

11. Kurabayashi K, Hirakawa Y, Tanimoto K, Tomita H, Hirakawa H. Identification of a second two-component signal transduction system that controls fosfomycin tolerance and glycerol-3- phosphate uptake. J. Bacteriol. 197:861-871, 2015

12. Kurushima J, Nakane D, Nishizaka T, Tomita H. Bacteriocin protein BacL1 of Enterococcus faecalis targets cell division loci and specifically recognizes L-Ala2-cross-bridged peptidoglycan. J. Bacteriol. 197:286-295, 2015

13. Kudo M, Nomura T, Yomoda S, Tanimoto K, Tomita H. Nosocomial infection caused by vancomycin-susceptible multidrug-resistant Enterococcus faecalis over a long term in a university hospital in Japan. Microbiol Immunol. 58:607-614, 2014

14. Kurabayashi K, Hirakawa Y, Tanimoto K, Tomita H, Hirakawa H. Role of the CpxAR two-component signal transduction system in control of fosfomycin resistance and carbon substrate uptake. J. Bacteriol. 196:248-256, 2014

15. Kurushima J, Hayashi I, Sugai M, Tomita H. Bacteriocin Protein BacL1 of Enterococcus faecalis is a peptidoglycan D-Isoglutamyl-L-lysine Endopeptidase. Journal of Biological Chemistry.; 288: 36915-36925, 2013

16. Tsutsumi Y, Tomita H, Tanimoto K. Identification of Novel Genes Responsible for Overexpression of ampC in Pseudomonas aeruginosa PAO1. Antimicrobial Agents Chemotherapy. 57:5987-5993, 2013

17. Nomura T, Tanimoto K, Shibayama K, Arakawa Y, Fujimoto S, Ike Y, and Tomita H.

Identification of VanN-Type Vancomycin Resistance in an Enterococcus faecium Isolate from Chicken Meat in Japan. Antimicrobial Agents and Chemotherapy. 56(12) :6389-6392,2012

18. Yamashita H, Tomita H, Inoue Y, and Ike Y. Genetic organization and mode of action of a novel bacteriocin, bacteriocin 51: determinant of vanA-type vancomycin-resistant Enterococcus faecium. Antimicrob Agents and Chemother. 55(9): 4352-4360, 2011.

19. Li Y, Tomita H, Lv Y, Liu J, Xue F, Zheng B, and Ike Y. Molecular characterization of erm(B)-and mef(E)-mediated erythromycin-resistant Streptococcus pneumoniae in China and complete DNA sequence of Tn2010. Journal of Applied Microbiology 110:254-265, 2010

20. Zheng B., Tomita H., Inoue T. and Ike Y. Isolation of VanB-Type Enterococcus faecalis Strains from Nosocomial Infections: First Report of the Isolation and Identification of the Pheromone-Responsive Plasmids pMG2200, Encoding VanB-Type Vancomycin Resistance and a Bac41-Type Bacteriocin, and pMG2201, Encoding Erythromycin Resistance and Cytolysin (Hly/Bac). Antimicrob Agents Chemother. 53(2): 735-747, 2009

21. Tomita H. and Ike Y. Genetic Analysis of the Enterococcus Vancomycin-Resistance Conjugative Plasmid pHTβ: Identification of the Region involved in Cell Aggregation and traB, a Key Regulator Gene for Plasmid Transfer and Cell Aggregation. J Bacteriol. 190(23): 7739-7753, 2008

22. Tanimoto K., and Ike Y. Complete nucleotide sequencing and analysis of the 65-kb highly conjugative Enterococcus faecium plasmid pMG1: identification of the transfer-related region and the minimum region required for replication. FEMS Microbiol Lett. 288: 186-195, 2008

23. Tanimoto K., Tomita H., Fujimoto S., Okuzumi K. and Ike Y. Fluoroquinolone enhances the mutation frequency for meropenem-selected carbapenem resistance in Pseudomonas aeruginosa, but use of the high-potency drug doripenem inhibits mutant formation. Antimicrob Agents Chemother. 52(10): 3795-3800, 2008

24. TomitaH, kamei E, Ike Y. Cloning and genetic analyses of the bacteriocin 41 determinant encoded on the Enterococcus faecalis pheromone-responsive conjugative plasmid pYI14: a novel bacteriocin complemented by two extracellular components (lysin and activator). J Bacteriol 190(6): 2075-85, 2008

25. Zheng B, Tomita H, Xiao YH, Ike Y. The first molecular analysis of clinical isolates of VanA-type vancomycin-resistant Enterococcus faecium strains in Mainland China. Letters in Applied Microbiology 45: 307-312, 2007

26. Zheng B, Tomita H, Xiao YH, Wang S, Li Y, Ike Y. Molecular characterization of vancomycin-resistant enterococcus faecium isolates from mainland China. J Clin Microbiol 45(9): 2813-8, 2007

27. Todokoro D, Tomita H, Inoue T, Ike Y. Genetic analysis of bacteriocin 43 of vancomycin-resistant Enterococcus faecium. Appl. Environ. Microbiol., 2006 Nov. 72(11) 6955-64

28. Lim SK, Tanimoto K, Tomita H, Ike Y. Pheromone-responsive conjugative vancomycin resistance plasmids in Enterococcus faecalis isolates from human and chicken feces. Appl. Environ. Microbiol., 2006 Oct. 72(10) 6544-53

29. Tanimoto K, Nomura T, Maruyama H, Tomita H, Shibata N, Arakawa Y, Ike Y. First VanD-type vancomycin-resistant Enterococcus raffinosus isolate. Antimcrob. Agents Chemother., 2006 Nov. 50(11) 3966-67

30. Shin E, Hong HG, Ike Y, Lee K, Park YH, Lee DT, Lee Y. VanB-vanA incongruent VRE isolated from animals and humans in 1999. J. Microbiol., 2006 Aug. 44(4) 453-6.

31. Wachino J, Yamane K, Kimura K, Shibata N, Suzuki S, Ike Y, Arakawa Y. Mode of transposition and expression of 16S rRNA methyltransferase gene rmtC accompanied by ISEcp1. Antimicrob. Agents Chemother., 2006 Sep. 50(9) 3212-5

32. Jung EK, Hong SK, Lim JY, Lim SK, Kwon NH, Kim JM, Koo HC, Kim SH, Seo KS, Ike Y, Tanimoto K, Park YH. Phenotypic and genetic characterization of vancomycin-resistant enterococci from hospitalized humans and from poultry in Korea. FEMS Microbiol. Lett., 2006 Jul. 260(2) 193-200

33. Sakyo S, Tomita H, Tanimoto K, Fujimoto S, Ike Y. Potency of carbapenems for the prevention of carbapenem- resistant mutants of Pseudomonas aeruginosa: The high potency of a new carbapenem doripenem(DRPM) J. Antibiot., 2006 Apr. 59(4) 220-8

34. Inoue T, Tomita H, Ike Y. Bac 32, a novel bacteriocin widely disseminated among clinical isolates of Enterococcus faecium. Antimicrob. Agents Chemother., 2006 Apr. 50(4)1202-12

35. Wachino J, Kurokawa H, Suzuki S, Yamane K, Shibata N, Kimura K, Ike Y, Arakawa Y. Horizontal transfer of blaCMY-bearing plasmids among clinical Escherichia coli and Klebsiella pneumoniae isolates and emergence of cefepime-hydrolyzing CMY-19. Antimicrob Agents Chemother. 2006 Feb;50(2):534-41.

36. Wachino J, Yamane K, Shibayama K, Kurokawa H, Shibata N, Suzuki S, Doi Y, Kimura K, Ike Y, Arakawa Y. Novel plasmid-mediated 16S rRNA methylase, RmtC, found in a proteus mirabilis isolate demonstrating extraordinary high-level resistance against various aminoglycosides. Antimicrob Agents Chemother. 2006 Jan;50(1):178-84.

37. Tomita H, Ike Y. Genetic analysis of transfer-related regions of the vancomycin resistance Enterococcus conjugative plasmid pHTβ: identification of oriT and a putative relaxase gene.J Bacteriol. 2005 Nov;187(22):7727-37.

38. Tanimoto K, Nomura T, Hamatani H, Xiao YH, Ike Y. A vancomycin-dependent VanA-type Enterococcus faecalis strain isolated in Japan from chicken imported from China. Lett Appl Microbiol. 2005;41(2):157-62.

39. Takeuchi K, Tomita H, Fujimoto S, Kudo M, Kuwano H, Ike Y. Drug resistance of Enterococcus faecium clinical isolates and the conjugative transfer of gentamicin and erythromycin resistance traits. FEMS Microbiol Lett. 2005 Feb 15;243(2):347-54.

40. Tomita H, Ike Y. Tissue-specific adherent Enterococcus faecalis strains that show highly efficient adhesion to human bladder carcinoma T24 cells also adhere to extracellular matrix proteins. Infect Immun. 2004 Oct;72(10):5877-85.

41. Arakawa Y, Ike Y, Nagasawa M. Where has vancomycin-heterogeneously resistant Staphylococcus aureus gone? Lancet. 2004 Apr 24;363(9418):1401.

42. Tomita H, Tanimoto K, Hayakawa S, Morinaga K, Ezaki K, Oshima H, Ike Y. Highly conjugative pMG1-like plasmids carrying Tn1546-like transposons that encode vancomycin resistance in Enterococcus faecium. J Bacteriol. 2003 Dec;185(23):7024-8.

43. Ozawa Y, Tanimoto K, Nomura T, Yoshinaga M, Arakawa Y, Ike Y. Vancomycin-resistant enterococci in humans and imported chickens in Japan. Appl Environ Microbiol. 2002 Dec;68(12):6457-61.

44. Tanimoto K, Ike Y. Analysis of the conjugal transfer system of the pheromone-independent highly transferable Enterococcus plasmid pMG1: identification of a tra gene (traA) up-regulated during conjugation. J Bacteriol. 2002 Oct;184(20):5800-4.

45. Tomita H, Pierson C, Lim SK, Clewell DB, Ike Y. Possible connection between a widely disseminated conjugative gentamicin resistance (pMG1-like) plasmid and the emergence of vancomycin resistance in Enterococcus faecium. J Clin Microbiol. 2002 Sep;40(9):3326-33.

46. Ike Y, Arakawa Y, Ma X, Tatewaki K, Nagasawa M, Tomita H, Tanimoto K, Fujimoto S. Nationwide survey shows that methicillin-resistant Staphylococcus aureus strains heterogeneously and intermediately resistant to vancomycin are not disseminated throughout Japanese hospitals. J Clin Microbiol. 2001 Dec;39(12):4445-51.

47. Francia MV, Haas W, Wirth R, Samberger E, Muscholl-Silberhorn A, Gilmore MS, Ike Y, Weaver KE, An FY, Clewell DB. Completion of the nucleotide sequence of the Enterococcus faecalis conjugative virulence plasmid pAD1 and identification of a second transfer origin. Plasmid. 2001 Sep;46(2):117-27.

48. Fujimoto S, Ike Y. pAM401-based shuttle vectors that enable overexpression of promoterless genes and one-step purification of tag fusion proteins directly from Enterococcus faecalis. Appl Environ Microbiol. 2001 Mar;67(3):1262-7.

49. Arakawa Y, Ike Y, Nagasawa M, Shibata N, Doi Y, Shibayama K, Yagi T, Kurata T. Trends in antimicrobial-drug resistance in Japan. Emerg Infect Dis. 2000 Nov-Dec;6(6):572-5.

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